Directed mutagenesis method for analysis of mutagen specificity: application to ultraviolet-induced mutagenesis.
نویسندگان
چکیده
منابع مشابه
Directed mutagenesis.
METHODS THAT GENERATE BASE SUBSTITUTIONS ...................................... Fragment Mutagenesis followed by Recombination .................................................. Site Mutagenesis of Intact Genomes ....................................... Incorporation of nucleotide analogues ............................. Bisulfte reaction at single-stranded gaps .....................................
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BACKGROUND: Among all common techniques in sitedirectedmutagenesis, λ Red recombinase system has beenwidely used to knock out chromosomal genes in bacteria. In thismethod, there is always the risk of DNA Linear digestion byhost's restriction enzymes that leads to the low frequency ofrecombination. OBJECTIVES:To overcome this, we constructeda recombinant vector to disrupt phoP gene in Salmonella...
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Promega's new GeneEditorTM in vitro Site-Directed Mutagenesis System* provides a high efficiency, reliable procedure for the generation and selection of oligonucleotide-directed mutants. The Selection Oligonucleotides provided in this system encode mutations which alter the substrate specificity of beta-lactamase, the gene encoding ampicillin resistance, commonly found on cloning vectors. This ...
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Mutagenesis is an important tool to study gene regulation, model disease-causing mutations and for functional characterisation of proteins. Most of the current methods for mutagenesis involve multiple step procedures. One of the most accurate methods for genetically altering DNA is recombineering, which uses bacteria expressing viral recombination proteins. Recently, the use of in vitro seamles...
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Structural elements of the rat 1.t-opioid receptor important in ligand receptor binding and selectivity were examined using a site-directed mutagenesis approach. Five single amino acid mutations were made, three that altered conserved residues in the ~ 8, and K receptors (Asn 15°t Ala, His297 to Ala, and Tyr326 to Phe) and wo de igned to test for ~i/8 selectivity (lie198 to Val and Va1202 to li...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.1.237